| Annotated protein: | Egl nine homolog 1 (EC 1.14.11.29) (Hypoxia-inducible factor prolyl hydroxylase 2) (HIF-PH2) (HIF-prolyl hydroxylase 2) (HPH-2) (Prolyl hydroxylase domain-containing protein 2) (PHD2) (SM-20). Gene symbol: EGLN1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q91YE3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EGLN1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Segura I, et al. "The Oxygen Sensor PHD2 Controls Dendritic Spines and Synapses via Modification of Filamin A" Cell Rep. 2016 Mar 22;14(11):2653-67 PMID:26972007 |
| Figure(s): | Fig.3 |
| Annotation description: | Fig.3A+B: "PHD2 Expression and Silencing in Dendritic Spines" - "IB showed that PHD2 was broadly present, including in synaptosomes and PSDs (Figure 3A). Using an antibody specific for PHD2 in immunostainings (Figures S4D-S4H), PHD2 was detected in neurites and dendritic protrusions of MHNs (Figure 3B) and rat hippocampal neurons (RHNs) (data not shown), in close apposition to synaptophysin+ puncta, suggesting postsynaptic localization. A wide distribution was also obtained in MHNs transfected with YFP-tagged PHD2 (PHD2WTYFP) (Figure S4I)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 1048 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |