| Annotated protein: | Adhesion G protein-coupled receptor L3 (Latrophilin-3) (Lectomedin-3). Gene symbol: ADGRL3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q80TS3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADGRL3 |
| Ontology domain: | Biological Process |
| SynGO term: | synapse organization (GO:0050808) |
| Synapse type(s): | neocortex, glutamatergic |
| Annotated paper: | O'Sullivan ML, et al. "LPHN3, a presynaptic adhesion-GPCR implicated in ADHD, regulates the strength of neocortical layer 2/3 synaptic input to layer 5" Neural Dev. 2014 Apr 17;9:7 PMID:24739570 |
| Figure(s): | Fig 1, Fig 2, Fig 3, Fig 5, Fig 6 |
| Annotation description: | Latrophilin-3 regulates the strength of synaptic input from L2/3 to L5 as shown by knockdown of latrophilin-3 in L2/3 neurons and electrophysiological measurements in acute slices (Fig. 1). Specifically, latrophilin-3 regulates strength of synaptic input, not by changing release probability (Fig. 2) but by controlling the density of synapses formed by L2/3 axons in L5 (synapse density in L5 was reduced when latrophilin3 was lost in L2/3 neurons; Fig. 3). The latrophilin-3 Olfactomedin domain was required for binding to FLRT3 (Fig. 5), and mutant latrophilin3 lacking the Olfactomedin domain was not capable of rescuing the deficit in presynaptic density following knockdown of endogenous latrophilin-3 (Fig. 6) |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Microscopy (generic) Whole-cell patch clamp Electrophysiology (generic) |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | Latrophilin-3 regulates glutamatergic synapse number: excess soluble ecto-LPHN3-Fc protein applied to dissociated hippocampal cultures to competitively disrupt endogenous LPHN3 complexes resulted in decreased number of glutamatergic synapses on dentate granule cells by immunofluorescence (Figure 3A-C), corroborated with culture-wide knockdown of LPHN3 that reduced the frequency of mEPSCs (Figure 3K) without affecting mEPSC amplitude (Figure 3L). @ PMID:22405201 |
| SynGO annotation ID: | 1044 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |