| Annotated protein: | Adenosine receptor A1. Gene symbol: ADORA1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P25099 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADORA1 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of presynaptic cytosolic calcium levels (GO:0099509) |
| Synapse type(s): | Calyx of Held |
| Annotated paper: | Kimura M, et al. "Adenosine A(1) receptor-mediated presynaptic inhibition at the calyx of Held of immature rats" J Physiol. 2003 Dec 1;553(Pt 2):415-26 PMID:12963795 |
| Figure(s): | Fig.1, Fig. 4 |
| Annotation description: | Activation of the G-protein coupled adenosine A1 receptor by adenosine causes an inhibition of neurotransmitter release by modulating presynaptic calcium currents. Principal cells in the MNTB in acute brainstem slices were analyzed by whole cell patch clamp electrophysiology and presynaptic action potentials were evoked by fiber stimulation. Bath application of adenosine caused an attenuation of EPSCs and the effect could be rescued by the application of the adenosine A1 receptor antagonist CPT (Fig.1) indicating that activation of A1 receptors results in an inhibition of neurotransmitter release. Whole-cell voltage-clamp recordings from presynaptic calyx of Held terminals in response to a strong depolarization pulse revealed that bath application of adenosine resulted in an inhibition of presynaptic calcium currents (Fig.4), indicating a clear presynaptic effect for A1 receptors in the calyx of Held synapse. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1042 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |