| Annotated protein: | Gamma-secretase subunit APH-1 (APH-1). Gene symbol: APH1A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q5PQQ3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ APH1A |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | brain |
| Annotated paper: | Frykman S, et al. "Synaptic and endosomal localization of active gamma-secretase in rat brain" PLoS One. 2010 Jan 28;5(1):e8948 PMID:20126630 |
| Figure(s): | 1,2 and 4 |
| Annotation description: | Aph-1 is a subunit of gamma secretase a heteroligomeric enzyme made up of at leat 4 membrane subunits: The catalytic subunit Presenilin 1 (or Presenilin 2) associated with nicastrin, Pen-2 and Aph-1. The paper uses density centrifugation (Fig 1) and subcellular fractionation (Fig 2 and Fig 4) to show the subunits distribute with each other evidencing a functional gamma secretase. This synaptic localised gamma secretase distribute to pre, post and synaptic vesicle membranes as evidenced by well characterized synaptic markers. This is consistent with localization but not selective enrichment in the synaptic sub-compartment. 27/10/2017 Pim - The data on Golgi and Rab5-positive endosome localization is not included since no synaptic context is shown for these compartments in this figure. - Although the biochemical fractionation does not go beyond 'synaptic membrane', the authors claim in Fig.6 that "The neurons showed extensive co-localization of γ-secretase and the pre-synaptic marker synapsin (Figure 6D), whereas the co-localization with the post-synaptic marker PSD-95 was rare (Figure 6E)." Together with the expert-disputed localization to SVs we chose the more specific term 'presynaptic plasma membrane'. - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1034 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |