| Annotated protein: | AP-2 complex subunit mu (AP-2 mu chain) (Adaptor protein complex AP-2 subunit mu) (Adaptor-related protein complex 2 subunit mu) (Clathrin assembly protein complex 2 mu medium chain) (Clathrin coat assembly protein AP50) (Clathrin coat-associated protein AP50) (Mu2-adaptin) (Plasma membrane adaptor AP-2 50 kDa protein). Gene symbol: AP2M1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P84092 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AP2M1 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Kim SH, et al. "Synaptic vesicle recycling at CNS snapses without AP-2" J Neurosci. 2009 Mar 25;29(12):3865-74 PMID:19321783 |
| Figure(s): | Fig 2, Fig 3, Fig 4 |
| Annotation description: | The authors use shRNA to knockdown (KD) AP-2 mu2 (gene AP2M1) in cultured rat hippocampal neurons. In figure 2B they use high intensity stimulation to load the neurons with FM4-64 (which is established to enter in synaptic vesicles (SVs). They wash and then stimulate exocytosis to destain the neurons (as the SVs undergo exocytosis, the FM4-64 dye is release into the culture media and looses fluorescence). Destaining in the KD is ~30% of that in control indicating a large decrease in the pool of recycling SVs. In figure 3 they examine the rates of endocytosis of 4 major SV proteins tagged with pHlourin. They stimulate exocytosis and then examine endocytosis of SVs post-stimulation. The rates of all 4 proteins are strongly decreased. In figure 4 they examine the rate of endocytosis of vGlutpHlourin during intense stimulation and measure a dramatic reduction. It is important to note, knockdown of AP-2 mu2 in this manuscript leads to loss of AP-2 alpha. Thus, it is not possible to distinguish between the function of these two proteins as they are obligate heterodimers. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Optical physiology |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| Additional literature: | This manuscript demonstrates that knockdown of AP-2 mu2 leads to loss of AP-2 alpha and that knockdown of AP-2 alpha leads to loss of AP-2 mu2. Thus, it is not possible to distinguish between loss of AP-2 mu2 and AP-2 alpha. They are obligate heterodimers. @ PMID:12952941 |
| SynGO annotation ID: | 1020 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |