| Annotated protein: | Cell adhesion molecule 1 (Immunoglobulin superfamily member 4) (IgSF4) (Nectin-like protein 2) (NECL-2) (Spermatogenic immunoglobulin superfamily) (SgIgSF) (Synaptic cell adhesion molecule) (SynCAM) (Tumor suppressor in lung cancer 1) (TSLC-1). Gene symbol: CADM1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8R5M8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CADM1 |
| Ontology domain: | Biological Process |
| SynGO term: | presynapse assembly (GO:0099054) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Biederer T, et al. "SynCAM, a synaptic adhesion molecule that drives synapse assembly" Science. 2002 Aug 30;297(5586):1525-31 PMID:12202822 |
| Figure(s): | Figs. 5, 6, 7 |
| Annotation description: | The experiments in Figs. 5 and 6 use a co-culture approach to demonstrate that the surface contact with the immunoglobulin adhesion protein SynCAM 1 is sufficient to induce neurons to assemble specializations that are positive for presynaptic markers and that contain recycling synaptic vesicles. Further, SynCAM 1-induced neuronal specializations release glutamate. These results support that SynCAM 1 is sufficient to induce neurons to form functional presynaptic terminals. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Electrophysiology (generic) Optical physiology |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | Using a co-culture approach, the results in Fig. 9 show that SynCAM 1 induces neurons to form presynaptic specializations and that it recruits neuronal SynCAM 2 to these sites. @ PMID:18003830 A role of SynCAM 1 in synapse and presynapse assembly in vivo is supported by the results in Fig. 2 showing that mice lacking SynCAM 1 have fewer excitatory synapses with shorter active zones and PSDs in the hippocampal CA1 area. Fig.3 shows that KO and overexpression of SynCAM 1 in mice decreases or increases excitatory transmission events, respectively, at Schaffer collaterals. @ PMID:21145003 In support of a role of SynCAM 1 in synapse assembly in vivo, the loss of SynCAM 1 in KO mice reduces the number of excitatory synapses in the hippocampal CA3 area. This includes a reduction in the number of excitatory synapses formed by mossy fibers onto CA3 pyramidal neurons (Fig. 3) and on PV interneurons (Fig. 4). @ PMID:27413156 |
| SynGO annotation ID: | 1018 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |