| Annotated protein: | Cell adhesion molecule 1 (Immunoglobulin superfamily member 4) (IgSF4) (Nectin-like protein 2) (NECL-2) (Spermatogenic immunoglobulin superfamily) (SgIgSF) (Synaptic cell adhesion molecule) (SynCAM) (Tumor suppressor in lung cancer 1) (TSLC-1). Gene symbol: CADM1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8R5M8 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CADM1 |
| Ontology domain: | Biological Process |
| SynGO term: | synapse adhesion between pre- and post-synapse (GO:0099560) |
| Synapse type(s): | forebrain |
| Annotated paper: | Fogel AI, et al. "SynCAMs organize synapses through heterophilic adhesion" J Neurosci. 2007 Nov 14;27(46):12516-30 PMID:18003830 |
| Figure(s): | Figs. 5, 6 |
| Annotation description: | The adhesive interactions of the synaptic adhesion molecule SynCAM 1 were mapped in this study. The homophilic binding of SynCAM 1 extracellular domains in trans is shown in Fig. 5, using a bead aggregation assay and a cell-cell contact analysis. Testing heterophilic interactions, the affinity chromatography results in Fig. 6A show that heterologously expressed SynCAM 1 retains not only SynCAM 1 but also SynCAM 2 from adult rat forebrain extracts. Beads coated with SynCAMs 2 and 3 each retain SynCAM 1 from forebrain extracts. Heterophilic interactions of SynCAM 1 and SynCAM 2 are further supported by the analysis of cell-cell contact assembly in Fig. 6B. All interactions are Ca2+ independent. |
| Evidence tracking, Biological System: | Intact tissue Non-neuronal tissue Cell-free system |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Protein-protein interaction (generic) |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| Additional literature: | Using a cell aggregation assay, the data in Fig.1 demonstrate the homophilic interaction of SynCAM1/nectin-like molecule 2. This is supported by a cell-cell contact analysis in Fig. 6. The heterophilic interaction of SynCAM 1 and SynCAM3/nectin-like molecule 1 is shown in Fig. 2. @ PMID:12826663 Using a cell overlay assay, Fig. 1 shows the heterophilic interaction of SynCAM 1 with SynCAM 2 and the heterophilic interactions of both SynCAM 2 and SynCAM 3 with SynCAM 1. @ PMID:18615557 The experiments shown in Fig. 4 use a cell overlay approach to show the homophilic interaction of SynCAM1/nectin-like molecule 2 and the heterophilic interaction of SynCAM 1 with SynCAM 3/nectin-like molecule 1. @ PMID:17724124 The homophilic binding of SynCAM 1 via its extracellular immunoglobulin domains is shown in Fig. 2B using a cell aggregation assay. @ PMID:12202822 |
| SynGO annotation ID: | 1016 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |