| Annotated protein: | Nuclear factor kappaB subunit p65 (RELA proto-oncogene, NF-kB subunit) (V-rel reticuloendotheliosis viral oncogene homolog A (Avian)). Gene symbol: RELA. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q7TQN4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RELA |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | cerebral cortex |
| Annotated paper: | Kaltschmidt C, et al. "Brain synapses contain inducible forms of the transcription factor NF-kappa B" Mech Dev. 1993 Oct;43(2-3):135-47 PMID:8297787 |
| Figure(s): | Fig 3a, b |
| Annotation description: | Authors use electrophoretic mobility shift assays (EMSAs) to detect the presence of kB-DNA-binding activities in the rat brain and rat synaptosomal preparations. They used a 32P-oligonucleoutide containing an NF-kB binding motif which was incubated with cell extracts and subjected to electrophoresis after the binding reaction to detect changes in motility. kB-binding activity was found in synaptosomal fractions with, but also without though less, treatment with DOC, which reveals latent kB-binding activities sequestered by IkB proteins. Previously, authors have described that p65/RelA complexes are responsible for kB-binding activities found in rat brain since incubation the IkB protein MAD3, which inhibits DNA-binding complexes containing p65 abolished the binding activities. The same effect was found when they used an antibody against the N terminus of p65, which also prevented DOC-activated complexes. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| SynGO annotation ID: | 1015 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |