| Annotated protein: | Heat shock cognate 71 kDa protein (EC 3.6.4.10) (Heat shock 70 kDa protein 8). Gene symbol: HSPA8. Taxonomy: Bos taurus (Bovine). Uniprot ID: P19120 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ HSPA8 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle uncoating (GO:0016191) |
| Annotated paper: | Morgan JR, et al. "Uncoating of clathrin-coated vesicles in presynaptic terminals: roles for Hsc70 and auxilin" Neuron. 2001 Oct 25;32(2):289-300 PMID:11683998 |
| Figure(s): | Fig. 1, Fig. 2, Fig. 3 |
| Annotation description: | The authors generate two peptides that bind to Hsc70, one from the co-chaperone auxilin and one from cytochrome C (although note a discrepancy in the species information). In Fig. 1 the authors demonstrate that the peptides block the uncoating of clathrin-coated vesicles (CCVs) in vitro. In figure 2 they inject the peptides into the squid (Loligo pealei) giant synapse. The peptides block neurotransmitter release measured physiologically. In Fig. 3, the authors demonstrate that the peptides block the uncoating of CCVs in the presynaptic nerve terminal. 14/8/2017 Pim - SV uncaging role of Hsc70 is tested in a reconstituted system using bovine clathrin and Hsc70 protein and recombinant auxilin protein (unkown species) using two Hsc70 blocking peptides. - Direct interaction of auxilin with bovine Hsc70 is shown with SPR. - Function of Hsc70 peptides is show with microinjection of squid neurons. |
| Evidence tracking, Biological System: | Intact tissue Cell-free system |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Electron Microscopy Electrophysiology (generic) Enzyme assay |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| Additional literature: | Hsc70 is a chaperone and auxilin is well established as a DNAJ domain-containing co-chaperone for auxilin in neurons. This paper demonstrates that hippocampal cultures from auxilin knockout mice have defects in CCV uncoating in the nerve terminal and in endocytosis of synaptic vesicles. The strong inference is that these phenotypes result from the inability of Hsc70 to be recruited to clatrhin coats in auxilin knockout neurons. @ PMID:20160091 |
| SynGO annotation ID: | 1014 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |