| Annotated protein: | Muscarinic acetylcholine receptor. Gene symbol: CHRM2. Taxonomy: Macaca Mulatta (Rhesus Macaque). Uniprot ID: A0A5F7ZKY2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CHRM2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic active zone membrane (GO:0099059) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Mrzljak L, et al. "Association of m1 and m2 muscarinic receptor proteins with asymmetric synapses in the primate cerebral cortex: morphological evidence for cholinergic modulation of excitatory neurotransmission" Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5194-8 PMID:8389473 |
| Figure(s): | 3 |
| Annotation description: | Paper shows immunohistological data support predominant presynaptic location of the mAChR-M2. Also indicates modest association in postsynaptic spines and dendrites. This work supports the wider view about predominant presynaptic signalling function of M2. Good evidence not unequivocal but best in the literature. 27/10/2017 Pim - Authors note that the stainig in the terminal is a bit shifted: "m2 immunoreactivity was localized predominantly in presynaptic axons (Figs. 1C and 3 C-H) and to a lesser extent postsynaptically in spines and small dendrites (Fig. 3 A and B).... A deceptive feature of the presynaptic labeling was that the immunoreaction product was commonly located in the axoplasm at some distance from presynaptic sites (Fig. 3 C-F)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| Additional literature: | Paper defines how antibody specificity is defined. @ PMID:1941081 |
| SynGO annotation ID: | 1013 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |