| Annotated protein: | Adenosine receptor A2a. Gene symbol: ADORA2A. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P30543 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADORA2A |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic modulation of chemical synaptic transmission (GO:0099171) |
| Synapse type(s): | striatum, glutamatergic |
| Annotated paper: | Rodrigues RJ, et al. "Co-localization and functional interaction between adenosine A(2A) and metabotropic group 5 receptors in glutamatergic nerve terminals of the rat striatum" J Neurochem. 2005 Feb;92(3):433-41 PMID:15659214 |
| Figure(s): | Fig.1,3,4 |
| Annotation description: | Treatment with the selective A2A receptor agonist CGS 21680 facilitates the depolarization (20mM K+)-dependent release of radiolabelled glutamate from isolated synaptosomes (Fig 1). The facilitatory effect was blocked by the selective A2A receptor antagonist SCH 5861, but not significantly by the A1 receptor antagonist DPCPX (Fig. 1). Subcellular fractionation of synaptosomes demonstrates the presence of A2A in presynaptic membranes (as well as predominately in postsynaptic membranes; Fig.3) and ICC using an antibody directed against A2A demonstrates a co-localization of A2A and synaptophysin and vGluT1 and 2 immunoreactivity (Fig.4). These data indicate a presynaptic function of A2A receptors in facilitating neurotransmitter release upon binding of adenosine. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist Antibody (detection) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Western blot Biochemical fractionation (generic) |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1011 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |