| Annotated protein: | Adenosine receptor A1. Gene symbol: ADORA1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P25099 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ADORA1 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic membrane (GO:0099055) |
| Synapse type(s): | medulla |
| Annotated paper: | Pickel VM, et al. "Subcellular distributions of adenosine A1 and A2A receptors in the rat dorsomedial nucleus of the solitary tract at the level of the area postrema" Synapse. 2006 Dec 1;60(7):496-509 PMID:16952160 |
| Figure(s): | Fig.1-6, Table 1 and 2. |
| Annotation description: | The location of the adenosine A1 receptor was assessed by pre-embedding immunoperoxidase and immunogold labelling. The A1 receptor was found in dendrites and spines (Table1 and 2, Fig.2-6). Immunoreactivity was found on plasma membranes, but also on endomembranes. An affinity purified polyclonal antiserum was raised against a synthetic rat peptide (aa309-326) in the C-terminus of the receptor. The specificity of the antibody was tested by Western Blot and IHC using wildtype and A1 KO mice (Fig.1). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1007 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |