| Annotated protein: | Src substrate cortactin. Gene symbol: CTTN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q66HL2 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CTTN |
| Ontology domain: | Biological Process |
| SynGO term: | modification of postsynaptic actin cytoskeleton (GO:0098885) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Hering H, et al. "Activity-dependent redistribution and essential role of cortactin in dendritic spine morphogenesis" J Neurosci. 2003 Dec 17;23(37):11759-69 PMID:14684878 |
| Figure(s): | Fig 1b,c,d; Fig 3a-g; Table 1; Fig 4; |
| Annotation description: | Authors used a pSuper-based siRNA construct to decrease contactin levels of primary hippocampal neurons and observed a significant decrease in the number of dendritic protrusions, suggesting that cortactin plays a role in spine formation or stability. The remaining spines were positive for F-actin and PSD95. By contrary, overexpression of cortactin induced an increase in spine length mainly attributable to increase in spine neck. Interestingly, this effect was mimicked by the overexpression of a cortactin mutant carrying only the NTA and tandem repeat domains of the protein, involved in actin polymerization. Overexpression of cortactin in aspiny inhibitory neurons also induced the small protrusions resembling filopodia, supporting the role of cortactin in spine formation. 11/8/2017 Pim - The data in this paper are rather corralational and strongly depend on the prior knowledge that "...cortactin binds to F-actin and affects actin polymerization...". However, the combined data on spine morphology and actin/cortactin localization, in my view, support the selected term 'regulation of modification of postsynaptic actin cytoskeleton (GO:1905274)'. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Maria Andres-Alonso (ORCID:0000-0002-1585-539X) Michael Kreutz (ORCID:0000-0003-0575-6950) |
| Lab: | RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany and Leibniz Group 'Dendritic Organelles and Synaptic Function', Center for Molecular Neurobiology, ZMNH, University MC, Hamburg-Eppendorf, Hamburg, 20251, Germany |
| Additional literature: | See Fig.3+4 (confocal and EM data). @ PMID:15548644 New paper detailing the role of Cortactin in F-actin dynamics. @ PMID:29478916 |
| SynGO annotation ID: | 1005 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |