| Annotated protein: | Catenin beta-1 (Beta-catenin). Gene symbol: CTNNB1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q02248 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CTNNB1 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle clustering (GO:0097091) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Bamji SX, et al. "Role of beta-catenin in synaptic vesicle localization and presynaptic assembly" Neuron. 2003 Nov 13;40(4):719-31 PMID:14622577 |
| Figure(s): | Figure 2A-F |
| Annotation description: | The paper makes use of a conditionally-floxed beta-catenin CKO mouse line. The conditional deletion is achieved via a CaMKII-cre transgenic allele (this results in a deletion at around P18). Figure 2: electron microscopy of stratum radiatum synapses shows: - a 25% increase in the number of excitatory, spine synapses (2A,B,D) in the CKO. - a 3-fold increase in perforated synapses (2C,E) in the CKO. - a 40% reduction in the undocked pool of vesicles per synapse (2F) in the CKO. - The number of docked vesicles located less than one vesicle diameter from the presynaptic membrane was similar (Figures 2A, 2B, and 2F). - The reduction in the number of vesicles per synapse in the CKO is not due to redistribution of vesicles over a larger number of synapses. Quantification of the total number of vesicles (in a defined region) shows a 30% reduction in the total number of vesicles. Taken together these data suggest a presynaptic role of beta-catenin in vesicle clustering at the reserve pool region adjacent to the active zone. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Noa Lipstein (ORCID:0000-0002-0755-5899) Cordelia Imig (ORCID:0000-0001-7351-8706) Vincent O'connor (ORCID:0000-0003-3185-5709) Nils Brose (ORCID:0000-0003-0938-8534) |
| Lab: | Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany |
| SynGO annotation ID: | 1001 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |